advancedbiomatrix TeloCol®-3說(shuō)明書(shū)
TeloCol®-3
TeloCol-3是50mL的3mg/mL的I型牛telocollagen溶液,用于2D涂層或3D水凝膠。該套件配有中和溶液,便于操作。TeloCol-3作為端羥基膠原,將形成更牢固的水凝膠,并且凝膠速度明顯快于相同濃度的端羥基膠原溶液。
產(chǎn)品描述
TeloCol®-3牛膠原蛋白溶液來(lái)源于酸提取過(guò)程,產(chǎn)生端肽完整膠原蛋白。TeloCol®是溶于0.01 N HCL的端羥基膠原。膠原鏈兩端的端肽區(qū),即N-端肽區(qū)和C-端肽區(qū)保持不變。
TeloCol®-3膠原蛋白約為95%的I型膠原蛋白,其余為III型膠原蛋白。每個(gè)產(chǎn)品包括一瓶含有50ml膠原蛋白溶液的瓶子,以及一瓶預(yù)先配制的用于形成膠原蛋白凝膠的中和溶液。該產(chǎn)品的供應(yīng)濃度約為3mg/ml。TeloCol®-3經(jīng)過(guò)無(wú)菌過(guò)濾,并以方便使用和儲(chǔ)存的包裝提供。
TeloCol®-3是表面涂層的理想選擇,為培養(yǎng)細(xì)胞提供薄層準(zhǔn)備,或用作固體凝膠。
Parameter, Testing, and Method | TeloCol®-3 Type I Collagen #5026 |
Sterilization Method | Filtration |
Extraction Method | Acid - telocollagen |
Form | Solution |
Package Size | 50 mL Kit |
Storage Temperature of Collagen | 2-10°C |
Storage Temperature of Neutralization Solution | Room Temperature |
Shelf Life | Minimum of 6 months from date of receipt |
Collagen Concentration - Biuret | 2.8-3.3 mg/mL |
Collagen Purity - Silver Staining | >99% |
pH | 1.9-3.0 |
Kinetic Gel Test (Minutes) | <40 |
Gel Formation Tube Test (Minutes) | <40 |
Fibrillogenesis (Absorbance Units) | >0.35 |
Electrophoretic Pattern - Coomassie Blue | Characteristic |
Sterility - USP modified | No growth |
Endotoxin - LAL | <10.0 EU/mL |
Osmolality (mOsmo H2O/kg) | <35 |
Cell Attachment Assay | Pass |
Source | Bovine Hide |
Hydrogel Young's Modulus E (Pa) | Characteristic |
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