現(xiàn)貨advancedbiomatrix PureCol®EZ凝膠說(shuō)明書(shū)
Catalog #5074
PureCol®EZ凝膠是一種5 mg/ml即用型牛去端膠原溶液,只需加熱至37°C即可形成3D水凝膠。該產(chǎn)品為pH中性,采用DMEM/F12培養(yǎng)基等滲配制,可立即添加細(xì)胞和3D水凝膠。
產(chǎn)品描述
PureCol®EZ凝膠是一種即用型膠原蛋白溶液,只需在培養(yǎng)箱中加熱至37°C即可形成牢固的凝膠。該產(chǎn)品由濃度為0.5%(約5mg/ml)的純化I型膠原、DMEM/F-12培養(yǎng)基和L-谷氨酰胺和二肽(L-丙氨酸-L-谷氨酰胺)的混合物組成,為細(xì)胞培養(yǎng)提供長(zhǎng)期的L-谷氨酰胺來(lái)源。
PureCol®EZ凝膠旨在通過(guò)預(yù)配制培養(yǎng)基并將產(chǎn)品調(diào)節(jié)至中性pH值來(lái)提高凝膠的稠度。該產(chǎn)品避免了因試劑添加、pH調(diào)節(jié)和處理?xiàng)l件的變化而導(dǎo)致的凝膠制備過(guò)程中的不一致。
PureCol®EZ凝膠是提供牢固凝膠的理想選擇,也可用于制備用于培養(yǎng)細(xì)胞的薄層。PureCol®EZ-Gel膠原蛋白采用用戶友好的包裝,便于使用和儲(chǔ)存。本產(chǎn)品是無(wú)菌的,作為即用溶液提供。
Parameter, Testing, and Method | PureCol® EZ Gel Type I Collagen #5074 |
Sterilization Method | Filtration |
Extraction Method | Enzyme - atelocollagen |
Form | Solution |
Package Size | 35 mL |
Storage Temperature | 2-10°C |
Shelf Life | Minimum of 6 months from date of receipt |
Collagen Concentration - Biuret | ~5 mg/mL |
Collagen Purity - Silver Staining | >99% |
pH | 6.9-7.4 |
Kinetic Gel Test (Minutes) | <40 |
Gel Formation Tube Test (Minutes) | <40 |
Fibrillogenesis (Absorbance Units) | >0.5 |
Electrophoretic Pattern - Coomassie Blue | Characteristic |
Sterility - USP modified | No growth |
Endotoxin - LAL | <1.0 EU/mL |
Osmolality (mOsmo H2O/kg) | 300-360 |
Cell Attachment Assay | Pass |
Source | Bovine Hide |
Hydrogel Young's Modulus E (Pa) | Characteristic |
Medium Supplement | DMEM/F-12 |
L-Glutamine Source | Mixture of L-Glutamine and Dipeptide (L-alanine-L-glutamine) |
電話
QQ咨詢
4006551678