advancedbiomatrix 用于3D水凝膠的RatCol®鼠尾膠原蛋白現(xiàn)貨
Catalog #5153
用于3D凝膠的RatCol®為25 mL 4 mg/mL,用于2D涂層或3D水凝膠的I型大鼠尾端膠原溶液。這款3D凝膠套件配有中和溶液,便于處理。作為端羥基膠原,用于3D凝膠的RatCol®將形成比端羥基膠原更牢固的水凝膠。
產(chǎn)品描述
RatCol®I型酸溶性大鼠尾巴膠原蛋白在0.02M乙酸溶液(~ pH 3)中含有100 mg,濃度約為4 mg/mL。RatCol®膠原蛋白是可溶性telo膠原蛋白。每個(gè)產(chǎn)品包括一瓶含有100mg膠原蛋白溶液的瓶子,以及一瓶預(yù)先配制的用于形成膠原蛋白凝膠的中和溶液。這款膠原蛋白產(chǎn)品采用方便用戶的包裝,便于使用和儲(chǔ)存。本產(chǎn)品經(jīng)過(guò)無(wú)菌過(guò)濾,作為即用溶液提供。
該產(chǎn)品非常適合于表面涂層,為培養(yǎng)細(xì)胞提供薄層制備,或用作固體凝膠。RatCol®膠原蛋白適用于使用多種細(xì)胞系的應(yīng)用,包括肝細(xì)胞、成纖維細(xì)胞和上皮細(xì)胞。
Parameter, Testing, and Method | RatCol® Type I Collagen #5153 |
Sterilization Method | Filtration |
Extraction Method | Acid - telocollagen |
Form | Solution |
Package Size | 100 mg (~25 mL) Kit |
Storage Temperature of Collagen | 2-10°C |
Storage Temperature of Neutralization Solution | Room Temperature |
Shelf Life | Minimum of 6 months from date of receipt |
Collagen Concentration - Biuret | 3.5-4.5 mg/mL |
Collagen Purity - Silver Staining | >99% |
pH | 3.0-3.8 |
Kinetic Gel Test (Minutes) | <40 |
Gel Formation Tube Test (Minutes) | <40 |
Electrophoretic Pattern - Coomassie Blue | Characteristic |
Sterility - USP modified | No growth |
Endotoxin - LAL | <10.0 EU/mL |
Osmolality (mOsmo H2O/kg) | <35 |
Cell Attachment Assay | Pass |
Source | Rat Tail Tendon |
Hydrogel Young's Modulus E (Pa) | Characteristic |
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